Part:BBa_K2958001:Design

Promoter->RBS->LacI->Double Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1209
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used a constitutive promoter to guarantee the production of Lac repressor protein in the absence of lactose or IPTG.

Source

The sources of this part are BBa_K608014 for the constitutive promoter and RBS, and BBa_K292005 for the Lac Repressor and double terminator.